Composite

Part:BBa_K5403019:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-02)


Fusion protein for RbsC-based membrane anchoring of GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 146
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in M. smegmatis.

Several modelling tools were used to aid in the design of this fusion protein (DeepTMHMM, Alphafold).


Source

M. smegmatis (RbsC) and Aequorea victoria

References